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Revista Brasileira de Plantas Medicinais

versão impressa ISSN 1516-0572

Resumo

CAMILLO, J.; SCHERWINSKI-PEREIRA, J.E.; VIEIRA, R.F.  e  PEIXOTO, J.R.. In vitro conservation of Cochlospermum regium (Schrank) pilg.- cochlospermaceae under minimal growth storage. Rev. bras. plantas med. [online]. 2009, vol.11, n.2, pp.184-189. ISSN 1516-0572.  http://dx.doi.org/10.1590/S1516-05722009000200012.

Cochlospermum regium is a plant from cerrado, caatinga and pantanal areas. In popular medicine, it is known as "algodão-do-campo" and its roots are used to treat uterine and intestinal infections, gastritis, ulcers and arthritis. Nowadays, extraction activities and the destruction of natural habitats has made "algodão-do-campo" one of the major native medicinal species for ex situ conservation. The aim of this work was to develop a methodology for the in vitro conservation of "algodão-do-campo", contributing to studies on the micropropagation of this species. In vitro germination was evaluated in "algodão-do-campo" seeds subjected to scarification or not with sulfuric acid and inoculation in MS medium. For in vitro conservation, nodal segments from in vitro-germinated seedlings were evaluated for 90 days at three temperatures (10, 20 and 25ºC) and three WPM medium concentrations (½, ¾ and full-strength). Scarified seeds presented 93.3% in vitro germination at 30 days of cultivation, a significantly higher value than the 13.3% observed for non-scarified seeds. The in vitro conservation of "algodão-do-campo" showed to be viable once cultures are kept in a growth chamber at 20ºC in ½ WPM medium. Under such conditions, the explants presented a minimal growth and 100% survival after three evaluation months.

Palavras-chave : "Algodão-do-campo"; in vitro conservation; micropropagation; germination; preservation; medicinal plants.

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