SciELO - Scientific Electronic Library Online

vol.15 issue2Quantification of total phenols present in the bark of Trichilia catigua A. Juss (Meliaceae)Pre-clinical toxicological evaluation of tea from the leaves of Morus nigra L. (Moraceae) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Revista Brasileira de Plantas Medicinais

Print version ISSN 1516-0572


ALVAREZ, A.S.; SANTOS, L.C.; ZOGHBI, M.G.B.  and  SILVA, R.J.F.. Evaluation of secretory structures of Ocimum gratissimum var. macrophyllum Briq. (Lamiaceae) after extraction of volatile compounds. Rev. bras. plantas med. [online]. 2013, vol.15, n.2, pp.237-243. ISSN 1516-0572.

The impact on the secretory structures of mature leaves of Ocimum gratissimum var. macrophyllum, after extraction of the volatile compounds using different extraction methods, was investigated by scanning electron microscopy (SEM). Cross-sections of the apex, the midrib and the margin showed surface with striated cuticle, diacitic stomata, single tector, filiform and glandular peltate trichomes. The peltate trichomes with a structure surrounded by cuticle containing the celllular exudate ranged from 60 to 80µm in diameter and consisted of a multicellular apical portion of radial arrangement formed by two concentric circles with 9 to 16 cells. In the central region of the peltate trichome apex, there were 4 cells in the form of a vertex arranged on the peduncle, and this was the most external region formed by a variable number of cells. The volatile compounds of the mature leaves were extracted by hydrodistillation (HD), simultaneous distillation-extraction (SDE) and solvent (SO). The obtained data evidenced that the major impact on the secretory structures occurred after extraction by hydrodistillation, compared to the other used methods. The percentage of the major chemical constituents, thymol and γ-terpinene, changed according the extraction method: (HD = 25.4%, SO = 18.2%, SDE = 54.8%) and γ-terpinene (HD = 38.2%, SO = absent, SDE = 13.7%).

Keywords : Ocimum gratissimum var. macrophyllum; Lamiaceae; medicinal plants; anatomical analysis.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License