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Revista Brasileira de Plantas Medicinais

versão impressa ISSN 1516-0572

Resumo

SANTI, M.M.; SANCHES, F.S.; SILVA, J.F.M.  e  SANTOS, P.M.L.. Phytochemical profile determination from extracts with antioxidant activity of the medicinal species of Cordia verbenacea DC. by HPLC-DAD. Rev. bras. plantas med. [online]. 2014, vol.16, n.2, pp.256-261. ISSN 1516-0572.  http://dx.doi.org/10.1590/S1516-05722014000200014.

The objective of the present study was to investigate the antioxidant activity of extracts from Cordia verbenacea leaves obtained by maceration in ethanol and partitioned with organic solvents. The infusion of leaves was also investigated. The total phenolic and total flavonoid content was evaluated by the Folin-Ciocalteau method and by the aluminum chloride complex method, respectively. The ethanol extract, the subfractions and the infusion were assayed at various concentrations to determine the DPPH scavenging activity expressed in terms of CE50. The best antioxidant activity was found on the ethyl acetate extract, EA, CE50 15.0 ± 0.5 µg.mL-1. The spectrophotometric assays revealed high phenol and flavonoid concentrations on the EA extract. The HPLC-DAD analysis was performed to reveal the UV-Vis profile of the chromatographic peaks of the EA extract. The UV spectral characteristics were related to phenol and polyphenol compounds.

Palavras-chave : Cordia verbenaceae; DPPH; phenols; flavonoids.

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