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Revista Brasileira de Plantas Medicinais

versão impressa ISSN 1516-0572versão On-line ISSN 1983-084X

Resumo

MATOS, D.O. et al. Determination of rosmarinic acid in Cordia verbenacea by liquid chromatography: applicability in seasonal study. Rev. bras. plantas med. [online]. 2015, vol.17, n.4, suppl.2, pp.857-864. ISSN 1516-0572.  http://dx.doi.org/10.1590/1983-084X/14_125.

In this study, a reverse phase-high performance liquid chromatography (RP-HPLC) technique for determination of rosmarinic acid in the Cordia verbenacea was developed and validated. A regression analysis was performed, with the observation of good linearity (r =0.999949). The values obtained for precision and accuracy determination are in agreement with ICH guidelines and the Brazilian legislation. The values of repeatability and intermediate precision were 2.79% and 4.76%, respectively. The detection and the quantitation limits of the rosmarinic acid were 1.92 µg/mL and 5.81 µg/mL, respectively. The results demonstrated that the developed method is a reliable RP-HPLC technique for the determination of rosmarinic acid in C. verbenacea tincture. In addition, this methodology was applied at a seasonal study indicating relatively strong positive correlation between the rain period and the rosmarinic acid content.

Palavras-chave : Cordia verbenacea; rosmarinic acid; HPLC.

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