SciELO - Scientific Electronic Library Online

 
vol.18 issue1  suppl.1Centesimal and mineral composition of medicinal plants commercialized in the Cuiabá Port Market, Mato Grosso, BrazilValidation of a rapid analytical method by HPLC for determining coumarin in guaco (Mikania glomerata Sprengel) confirmed with mass spectrometry author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Revista Brasileira de Plantas Medicinais

Print version ISSN 1516-0572On-line version ISSN 1983-084X

Abstract

SILVA, A.L.L. et al. Evaluation of antibacterial, cytotoxic, and antioxidant activity of the plant species Opuntia cochenillifera (L.) Mill. Rev. bras. plantas med. [online]. 2016, vol.18, n.1, suppl.1, pp.307-315. ISSN 1516-0572.  http://dx.doi.org/10.1590/1983-084X/15_145.

The purpose of this study was to evaluate the antibacterial, antioxidant, and cytotoxic activity of Opuntia cochenillifera (L.) Mill. A phytochemical screening and infrared (IR) absorption spectroscopy were performed in the crude ethanolic extracts and fractions of large and small cladodes. The antioxidant activity was evaluated through the qualitative method of free-radical scavenging capacity using the synthetic radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The cytotoxic activity was obtained by the cell viability assay using methyl thiazolyl tetrazolium (MTT). Antibacterial activity was evaluated by broth microdilution method to determine the minimum inhibitory concentration (MIC) against the bacterial strains Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli. The phytochemical screening mainly revealed the presence of phenols, flavonoids, free steroids, alkaloids, alkanes, and other chemical classes. The IR spectroscopy presented functional groups such as alkanes, carbonyls, methyl groups, carbon double bonds, and alkylamino groups, among others. Regarding cytotoxicity in the concentration of 100 μg/mL, neither the crude extracts, the fractions of the large cladode, nor the chloroform and methanol fractions of small cladode presented toxicity. The crude ethanolic extracts and fractions of large and small cladode showed no antibacterial or antioxidant activity. These results may provide support for future research aimed at other biological activities of this plant species.

Keywords : Opuntia cochenillifera (L.) Mill.; chemical composition; antibacterial activity; cytotoxic activity; antioxidant.

        · abstract in Portuguese     · text in Portuguese     · Portuguese ( pdf )