SciELO - Scientific Electronic Library Online

vol.118 issue4Environmental tobacco smoke exposure among non-smoking waiters: measurement of expired carbon monoxide levelsAntidepressant efficacy of sertraline and imipramine for the treatment of major depression in elderly outpatients author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Sao Paulo Medical Journal

Print version ISSN 1516-3180On-line version ISSN 1806-9460


GUATURA, Sandra Baltazar; MARTINEZ, José Antônio Baddini; BUENO, Patricia Cincotto dos Santos  and  SANTOS, Manuel Lopes dos. Increased exhalation of hydrogen peroxide in healthy subjects following cigarette consumption. Sao Paulo Med. J. [online]. 2000, vol.118, n.4, pp.93-98. ISSN 1516-3180.

CONTEXT: Increased hydrogen peroxide has been described in the expired breath condensate (H2O2-E) of several lung conditions, such as acute respiratory distress syndrome, chronic obstructive pulmonary disease and asthma. This technique has been advocated as being a simple method for documenting airway inflammation. OBJECTIVE: To evaluate H2O2-E in healthy cigarette smokers, and to determine the acute effects of the consumption of one cigarette on H2O2-E levels. TYPE OF STUDY: Prospective, controlled trial. SETTING: A pulmonary function laboratory in a University Hospital. PARTICIPANTS: Two groups of healthy volunteers: individuals who had never smoked (NS; n=10; 4 men; age = 30.6 ± 6.2 years) and current cigarette smokers (S; n=12; 7 men; age = 38.7 ± 9.8). None of the volunteers had respiratory symptoms and all showed normal spirometric tests. INTERVENTION: Expired air was collected from all volunteers through a face mask and a plastic collecting system leading into a flask with dry ice and pure ethanol. Samples from the group S were collected twice, before and half an hour after the combustion of one cigarette. MAIN MEASUREMENTS: Expired hydrogen peroxide using the Gallati and Pracht method. RESULTS: The S and NS groups showed comparable levels of H2O2-E at basal conditions [NS = 0.74 mM (DP 0.24) vs. S = 0.75 mM (DP 0.31)]. The smokers showed a significant increase in H2O2-E levels half an hour after the consumption of only one cigarette [0.75 mM (DP 0.31) vs. 0.95 mM (DP 0.22)]. CONCLUSION: The present results are consistent with the concept that smokers increase oxidative stress with elevated production of reactive oxygen species, contributing to the development of smoking-related disorders.

Keywords : Hydrogen peroxide; Smoking; Free radicals.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License