Revista Brasileira de Zootecnia
On-line version ISSN 1806-9290
MURGAS, Luis David Solis; FRANCISCATTO, Renan Toledo and SANTOS, Anna Graciela Oliveira. Sperm evaluation of piracanjuba fish (Brycon orbignyanus, Vallenciennes, 1849), after thawing. R. Bras. Zootec. [online]. 2003, vol.32, n.6, suppl.2, pp.1810-1814. ISSN 1806-9290. http://dx.doi.org/10.1590/S1516-35982003000800002.
The aim of this experiment was to test the effects of two extenders (Glucose 5% and Coconut Water) and two thawing rates (60ºC during 5 seconds and 50ºC during 10 seconds) for semen of Piracanjuba (Brycon orbygnianus, Vallencienens, 1849). A solution of NaHCO3 1% was used to activate motility. A factorial 2 x 2 was used to analyze the results on sperm motility and its duration, and the percentage of living sperm after thawing. The highest sperm motility values (74.17 ± 22.45%) were achieved when the combination of coconut water and thawing rates of 50ºC during 10 seconds was used. The other combinations of extender and thawing rates produced lower sperm motility. The sperm motility duration was not affected by any of the combinations of extenders and thawing rates. The thawing rates showes the average of 153.33 ± 59.61 seconds. The highest percentage of living sperm values (67.83 ± 17.67%) was obtained when the combination of glucose 5% and thawing rates of 50ºC during 10 seconds was used. This combination was more effective on the preservation of sperm piracanjuba integrity after freezing.
Keywords : Brycon orbygnianus; criopreservation; semen.