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Revista Brasileira de Zootecnia

Print version ISSN 1516-3598On-line version ISSN 1806-9290

Abstract

OLIVINDO, Cellyneude de Souza et al. Detection of Staphylococcus aureus using the REP-PCR technique to monitor goat milk quality. R. Bras. Zootec. [online]. 2009, vol.38, n.7, pp.1317-1321. ISSN 1806-9290.  https://doi.org/10.1590/S1516-35982009000700022.

The objective of the present study was to apply REP-PCR sequences in the monitoring goat milk quality, by detecting Staphylococcus aureus, in samples of from milker hands, goat teats, milk, milking machine and water, for the future establishment and implantation of the system of Hazard Analysis Critical Control Points (HACCP). Several fingerprints were verified of all the isolates collected from the different sources studied (milker hands, goat teats, milk, milking machine and water). Very similar band behaviors were observed that indicated that the isolates can be reported as epidemic clones. Milker´s hands were was characterized as a critical control point (CCP), because it stands out as an initial point of contaminationin the Staphylococcus aureus samples. The technique was shown to be efficient for the similarity analysis among individuals of the Staphylococcus aureus species, and is therefore a useful tool for investigation of management faults and consequently, in the search for more efficient control to prevent or minimize the spread of pathogenic microorganisms that cause serious illnesses in humans and animals that can be transmitted through products such as milk and its products.

Keywords : fingerprints; management; molecular epidemiology.

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