Revista Brasileira de Zootecnia
versão On-line ISSN 1806-9290
FAGUNDES, Bruno et al. Adding insulin to frozen seminal extender from Mangalarga Marchador stallions. R. Bras. Zootec. [online]. 2010, vol.39, n.2, pp. 273-278. ISSN 1806-9290. http://dx.doi.org/10.1590/S1516-35982010000200007.
Success rates in equine sperm cryopreservation are lower than in other domestic species. The objective of this study was to verify the effect of adding 0.1 IU/mL, 1 IU/mL and 10 IU/mL insulin to conventional frozen extender using computerized analysis of sperm motility (CASA), plasma membrane functionality by hypo-osmotic shock and acrossomal membrane integrity through the FITC/PSA assay. No difference was observed in treatment with 0.1 and 1 IU/mL after thawing in the analysis of motility and kinematic sperm parameters. However, when 10 IU/mL insulin was used there was a reduction in these parameters.
Palavras-chave : equine; freezing; plasmatic membrane; sperm.