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Revista Brasileira de Zootecnia

On-line version ISSN 1806-9290


SOUZA, Luiz Waldemar de Oliveira et al. Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay. R. Bras. Zootec. [online]. 2011, vol.40, n.12, pp.2721-2724. ISSN 1806-9290.

The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R2 = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

Keywords : bull; hormone; RIA; semen; spermatozoa; testosterone.

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