STAT1
|
C/T |
314 |
F: 5′GCCTCAAGTTTGCCAGTGGC3′ R: 5′GGCTCCCTTGATAGAACTGT3′ |
95°C 5′ (94 °C 45s, 63°C to 50°C, −2°C per cycle, 45s, 72°C 45s) 32 cycles, 72°C 10′ |
BspH I |
Rychtářová et al. (2014)Rychtářová, J.; Sztankóová, Z.; Kyselová, J.; Zink, V.; Štípková, M.; Vacek, M. and Štolc, L. 2014. Effect of DGAT1, BTN1A1, OLR1, and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed. Czech Journal of Animal Science 59:45-53. https://doi.org/10.17221/7228-CJAS
https://doi.org/10.17221/7228-CJAS...
|
OLR1
|
A/C |
143 |
F: 5′TCCCTAACTTGTTCCAAGTCCT3′ R: 5′CTCTACAATGCCTAGAAGAAAGC3′ |
94°C 5′ (94 °C 30s, 62°C 30s, 72°C 40s) 30 cycles, 72°C 5′ |
Pst I |
Komisarek and Dorynek (2009)Komisarek, J. and Dorynek, Z. 2009. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. Journal of Applied Genetics 50:125-132. https://doi.org/10.1007/BF03195663
https://doi.org/10.1007/BF03195663...
|
CSN1S1
|
B/C |
344 |
F: 5′ACAATTCTACCAGCTGGATGCCTATC3′ R: 5′CACGCTCCACAGTTCCTGAGTAA3′ |
94°C 3′ (94 °C 30s, 63°C 45s, 72°C 1′) 40 cycles, 72°C 10′ |
Hph I |
Kucerova et al. (2006)Kucerova, J.; Matejicek, A.; Jandurova, O.; Sorensen, P.; Nemcova, E.; Stipkova, M.; Kott, T.; Bouska, J. and Frelich, J. 2006. Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh. Czech Journal of Animal Science 51:241-247. https://doi.org/10.17221/3935-CJAS
https://doi.org/10.17221/3935-CJAS...
|
CSN1S2
|
D/A |
356 |
F: 5′AAAACAAGCAGCCAAGAAGC3′ R: 5′TTCCCAGTCTCCCCAGTATG3′ |
94°C 2′ (94 °C 30s, 60.5°C 30s, 72°C 1′) 30 cycles, 72°C 10′ |
Mnl I |
Ibeagha-Awemu et al. (2007)Ibeagha-Awemu, E.; Prinzenberg, E. M.; Jann, O.; Lühken, G.; Ibeagha, A.; Zhao, X. and Erhardt, G. 2007. Molecular characterization of bovine CSN1S2* B and extensive distribution of zebu-specific milk protein alleles in European cattle. Journal of Dairy Science 90:3522-3529. https://doi.org/10.3168/jds.2006-679
https://doi.org/10.3168/jds.2006-679...
|
DGAT1
|
A/K |
411 |
F: 5′GCACCATCCTCTTCCTCAAG3′ R: 5′GGAAGCGCTTTCGGATG3′ |
94°C 4′, 10 cycles (94°C 60 s, 66°C 60s; -1°C per cycle, 72°C 60s), 25 cycles (94°C 60s, 56 °C 120 s, 72°C 60s), 72°C 15′ |
Cfr I |
Lacorte et al. (2006)Lacorte, G.; Machado, M.; Martinez, M.; Campos, A.; Maciel, R.; Verneque, R.; Teodoro, R.; Peixoto, M.; Carvalho, M. and Fonseca, C. 2006. DGAT1 K232A polymorphism in Brazilian cattle breeds. Genetics and Molecular Research 5:475-482.
|