Revista Brasileira de Ciência Avícola
Print version ISSN 1516-635X
On-line version ISSN 1806-9061
OLIVEIRA, GH; BERCHIERI JR, A and MONTASSIER, HJ. Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa. Rev. Bras. Cienc. Avic. [online]. 2006, vol.8, n.1, pp.51-54. ISSN 1516-635X. http://dx.doi.org/10.1590/S1516-635X2006000100008.
This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
Keywords : Chicken; ELISA; enzyme; Salmonella sp; serology.