Revista Brasileira de Hematologia e Hemoterapia
versión impresa ISSN 1516-8484
GIOVELLI, Letícia L. et al. Determination of the accuracy of the measurement method for dehydrogenase activity. Rev. Bras. Hematol. Hemoter. [online]. 2007, vol.29, n.4, pp. 378-381. ISSN 1516-8484. http://dx.doi.org/10.1590/S1516-84842007000400011.
Glucose-6-phosphate dehydrogenase deficiency (G6PD) is a public health problem which affects about 400 millions of people all over the world. Some methods that measure the activity of G6PD have already been developed. Thus, the aim of this study was to evaluate the accuracy of the Brewer's method compared with a standard reference and estimate the prevalence of G6PD deficiency in the sample. A cross-sectional study of a group of patients in HCPA presenting with jaundice was carried out from June 2004 to May 2005. Samples were processed by the metahaemoglobin reduction test (Brewer's method) and by the method of Haemoglobin Normalization, which was used as the standard reference. A total of 173 patients were analyzed for G6PD activity. The ages varied from one day to 82 years old with 66% of the sample being less than 16 days old. The mean activity and standard deviation of G6PD for the analyzed sample was 17.67 ± 5.66 U/gHb. The estimated frequencies of G6PD deficiency for the standard reference and Brewer's method were 13 (7.7%) subjects (total or partial deficiency) and 14 (8.67%), respectively. When the Brewer's method was compared with the quantitative method of Hemoglobin Normalization, it showed a sensitivity of 92.8% and specificity of 98.7%. As G6PD deficiency predominates in our society, low cost tests, such as the Brewer's test can be used for screening this deficiency, mainly to monitor newborn babies who are at risk of developing jaundice.
Palabras llave : G6PD; sensitivity; specificity; Brewer's method; jaundice.