Interleukin-10 haplotypes are not associated with acute cerebral ischemia or high-risk transcranial Doppler in a newborn cohort of 395 children with sickle cell anemia

Belisário, André Rolim; Sales, Rahyssa Rodrigues; Toledo, Nayara Evelin; Velloso-Rodrigues, Cibele; Silva, Célia Maria; Viana, Marcos Borato

doi:10.1016/j.bjhh.2016.09.017

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Original Articles • Rev. Bras. Hematol. Hemoter. 39 (2) • Apr-Jun 2017 • https://doi.org/10.1016/j.bjhh.2016.09.017 copy

Interleukin-10 haplotypes are not associated with acute cerebral ischemia or high-risk transcranial Doppler in a newborn cohort of 395 children with sickle cell anemia

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

Background:

The etiology of stroke, a severe complication of sickle cell anemia, involves inflammatory processes. However, the pathogenetic mechanisms are unknown. The aim of this study was to evaluate the influence of interleukin-10 polymorphisms and haplotypes on the risk of acute cerebral ischemia and high-risk transcranial Doppler in 395 children with sickle cell anemia from the state of Minas Gerais, Brazil.

Methods:

Interleukin-10 haplotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and sequencing. The outcomes studied were acute cerebral ischemia and high-risk transcranial Doppler. Clinical data were retrieved from the children's records.

Results:

There was no statistically significant difference in the frequencies of polymorphisms and haplotypes between children with and without acute cerebral ischemia or children with or without high-risk transcranial Doppler. These data are consistent with a previous report that showed an absence of association between interleukin-10 plasma levels and high-risk transcranial Doppler velocity in children with sickle cell anemia.

Conclusion:

Interleukin-10 haplotypes were not associated with the risk of acute cerebral ischemia or high-risk transcranial Doppler velocity in children with sickle cell anemia from the state of Minas Gerais, Brazil.

Keywords:
Sickle cell anemia; Stroke; Polymorphism; Interleukin-10; Transcranial Doppler ultrasound

	Acute cerebral ischemia
Polymorphism	Yes (n = 26)	No (n = 369)	Total (n = 395^f f n = 394 for IL10 haplotypes because one child had an unclassifiable haplotype (GCC/GTA). )	p-Value	Odds ratio	95% CI
IL10 rs1800896 (A or G) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
AA	9 (5.6%)	152 (94.4%)	161 (100%)	0.503^a a X2 linear-by-linear association.
Ag	13 (7.1%)	170 (92.9%)	183 (100%)	0.544^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	1.32	0.58-3.05
gg	4 (7.8%)	47 (92.2%)	51 (100%)	0.704^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	1.25	0.41-3.77

IL10 rs1800871 (C or T) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
CC	12 (7.4%)	150 (92.6%)	162 (100%)	0.297^a a X2 linear-by-linear association.
Ct	13 (7.0%)	173 (93%)	186 (100%)	0.681^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	0.80	0.36-1.78
tt	1 (2.1%)	46 (97.9%)	47 (100%)	0.342^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	0.28	0.04-2.12

IL10 rs1800872 (A or C) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
CC	12 (7.4%)	150 (92.6%)	162 (100%)	0.297^a a X2 linear-by-linear association.
Ca	13 (7%)	173 (93%)	186 (100%)	0.681^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	0.80	0.36-1.78
aa	1 (2.1%)	46 (97.9%)	47 (100%)	0.342^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	0.28	0.04-2.12

IL10 haplotypes
High	4 (8%)	46 (92%)	50 (100%)	0.428^a a X2 linear-by-linear association.
Intermediate	13 (7.3%)	165 (92.7%)	178 (100%)
Low	9 (4.8%)	157 (93.4%)	166 (100%)

	High-risk TCD
Polymorphism	Yes (n = 29)	No (n = 309)	Total (n = 338^f f n = 337 for IL10 haplotypes because one child had an unclassifiable haplotype (GCC/GTA). )	p-Value	Odds ratio	95% CI
IL10 rs1800896 (A or G) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
AA	7 (5.1%)	130 (94.9%)	137 (100%)	0.151^a a X2 Linear-by-linear association.
Ag	18 (11.5%)	139 (88.5%)	157 (100%)	0.075^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	2.28	0.95-5.50
gg	4 (9.1%)	40 (9.1%)	44 (100%)	0.78^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	1.08	0.36-3.25

IL10 rs1800871 (C or T) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
CC	15 (10.7%)	125 (89.3%)	140 (100%)	0.311^a a X2 Linear-by-linear association.
Ct	11 (7.0%)	146 (93%)	157 (100%)	0.244^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	0.63	0.30-1.36
tt	3 (7.3%)	38 (92.7%)	41 (100%)	1.00^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	0.82	0.24-2.85

IL10 rs1800872 (A or C) ^e e Minor alleles, as recorded by dbSNP, are represented by lowercase letters.
CC	15 (10.7%)	125 (89.3%)	140 (100%)	0.311^a a X2 Linear-by-linear association.
Ca	11 (7.0%)	146 (93%)	157 (100%)	0.244^b b Fisher's exact test. ^, ^c c Dominant association (XX versus Xx or xx).	0.63	0.30-1.36
aa	3 (7.3%)	38 (92.7%)	41 (100%)	1.00^b b Fisher's exact test. ^, ^d d Recessive association (XX or Xx versus xx).	0.82	0.24-2.85

IL10 haplotypes
High	4 (9.3%)	39 (90.7%)	43 (100%)	0.198^a a X2 Linear-by-linear association.
Intermediate	17 (11.2%)	135 (88.8%)	152 (100%)
Low	8 (5.6%)	134 (94.4%)	142 (100%)

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[1] *Corresponding author at: Centro de Tecidos Biológicos de Minas Gerais, Fundação Hemominas, Rua das Goiabeiras, 779, 33400-000 Lagoa Santa, Minas Gerais, Brazil. E-mail address: andrebelisario@yahoo.com.br (A.R. Belisário).