Brazilian Archives of Biology and Technology
versión impresa ISSN 1516-8913
AGUILAR, Cristóbal; CHRISTOPHER, Augur; VINIEGRA-GONZALEZ, Gustavo y FAVELA, Ernesto. A comparison of methods to determine tannin acyl hydrolase activity. Braz. arch. biol. technol. [online]. 1999, vol.42, n.3, pp. 355-362. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89131999000300014.
Six methods to determine the activity of tannase produced by Aspergillus niger Aa-20 on polyurethane foam by solid state fermentation, which included two titrimetric techniques, three spectrophotometric methods and one HPLC assay were tested and compared. All methods assayed enabled the measurement of extracellular tannase activity. However, only five were useful to evaluate intracellular tannase activity. Studies on the effect of pH on tannase extraction demonstrated that tannase activity was considerably under-estimated when its extraction was carried out at pH values below 5.5 and above 6.0. Results showed that the HPLC technique and the modified Bajpai and Patil methods presented several advantages in comparison to the other methods tested.
Palabras llave : Tannin acyl hydrolase; assay; polyurethane foam; solid state fermentation; tannase.