Brazilian Archives of Biology and Technology
versão impressa ISSN 1516-8913
PRABHU, G. Nagendra e CHANDRASEKARAN, M. Purification and characterization of an anti-cancer enzyme produced by marine Vibrio Costicola under a novel solid state fermentation process. Braz. arch. biol. technol. [online]. 1999, vol.42, n.3, pp. 363-368. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89131999000300015.
L - Glutaminase, a therapeutically and industrially important enzyme, was produced from marine Vibrio costicola by a novel solid state fermentation process using polystyrene beads as inert support. The new fermentation system offered several advantages over the conventional systems, such as the yield of leachate with minimum viscosity and high specific activity for the target product besides facilitating the easy estimation of biomass. The enzyme thus produced was purified and characterised. It was active at physiological pH, showed high substrate specificity towards L - glutamine and had a Km value of 7.4 x 10-2 M. It also exhibited high salt and temperature tolerance indicating good scope for its industrial and therapeutic applications.
Palavras-chave : L- Glutaminase; Vibrio costicola; solid state fermentation; inert support; purification and characterisation.