Brazilian Archives of Biology and Technology
versão On-line ISSN 1678-4324
MELLO, Marcia O.; MELO, Murilo e APPEZZATO-DA-GLORIA, Beatriz. Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe. Braz. arch. biol. technol. [online]. 2001, vol.44, n.2, pp. 197-203. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132001000200014.
Callus was induced from root segments taken from in vitro grown plants of Curcuma zedoaria Roscoe. The explants were cultured on agar-solidified Murashige and Skoog medium supplemented with 13.4mM of a-naphthaleneacetic acid and 2.2mM of 6-benzylaminopurine at 25ºC in the dark. Histological analysis revealed that callus was formed from the hypertrophied cortical parenchyma cells of the explant. Some of these cells underwent division while the surrounding cells accumulated starch. Callus was capable of shoot bud regeneration after 70 days when it was transfered to liquid medium of the same composition. After 30 days in liquid medium, buds developed from nodular structures. The adventitious shoots developed extensive root systems when they were placed on agar-solidified Murashige and Skoog medium without growth regulators at 25º C in the light. The establishment of these plantlets in soil was about 95%.
Palavras-chave : Adventitious bud; anatomy; morphogenesis; Zingiberaceae.