Abstract

QUEIROZ SOUSA, Maria de Fátima Vieira de; LOPES, Carlos Edison  and  PEREIRA JUNIOR, Nei. A chemically defined medium for production of actinomycin D by Streptomyces parvulus. Braz. arch. biol. technol. [online]. 2001, vol.44, n.3, pp. 227-231. ISSN 1516-8913.  http://dx.doi.org/10.1590/S1516-89132001000300002.

A chemically defined medium consisting of D(+)fructose, L(-)threonine, K₂HPO₄, MgSO₄.7H₂O, ZnSO₄.7H₂O, CaCl₂.2H₂O, FeSO₄.7H₂O and deionized water, was developed to maximize the synthesis of actinomycin D by the Streptomyces parvulus DAUFPE 3124 strain. This medium resulted in the maximum antibiotic concentration of 133mg/L while using the original medium the production of actinomycin D was poor not surpassing 43mg/L.

Keywords : Actinomycin D; production; Streptomyces. parvulus; synthetic medium.