Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913
QUEIROZ SOUSA, Maria de Fátima Vieira de; LOPES, Carlos Edison and PEREIRA JUNIOR, Nei. A chemically defined medium for production of actinomycin D by Streptomyces parvulus. Braz. arch. biol. technol. [online]. 2001, vol.44, n.3, pp. 227-231. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132001000300002.
A chemically defined medium consisting of D(+)fructose, L(-)threonine, K2HPO4, MgSO4.7H2O, ZnSO4.7H2O, CaCl2.2H2O, FeSO4.7H2O and deionized water, was developed to maximize the synthesis of actinomycin D by the Streptomyces parvulus DAUFPE 3124 strain. This medium resulted in the maximum antibiotic concentration of 133mg/L while using the original medium the production of actinomycin D was poor not surpassing 43mg/L.
Keywords : Actinomycin D; production; Streptomyces. parvulus; synthetic medium.