A method for the evaluation of arsenic and selenium in soluble coffee by inductively coupled plasma atomic emission spectrometry with continuous hydride generation to attend the Brazilian food legislation is described. Samples were digested with nitric acid and hydrogen peroxide in a focused microwave system. Slow heating eliminated nitric acid and selenium (VI) was reduced to selenium (IV) by addition of 6 mol/L hydrochloric acid and heating at 90° C under a reflux system. The influence of sample acidity on sensitivity was investigated. Hydrochloric acid 6 mol/L was the most suitable reaction medium. Practical detection limits of 2.0mug/L for As and 1.0mu g/L for Se were achieved and attended the Brazilian food legislation. The results of recoveries on spiked samples demonstrate the reliability and accuracy of the procedure.
ICP-AES; As; Se; soluble coffee
