Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913
HASENACK, Beatriz S. et al. The effect of concanavalin A on the replication of rotavirus (SA-11) in cell culture. Braz. arch. biol. technol. [online]. 2002, vol.45, n.2, pp. 125-135. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132002000200003.
Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used for monitoring the experiments. It was concluded that RV replication was not affected consistently by ConA, however it interfered with the development of cytopathic effect (CPE) without altering virus yields.
Keywords : Rotavirus; Concanavalin A; Cell culture.