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Brazilian Archives of Biology and Technology
On-line version ISSN 1678-4324
FERRAZ, Eduardo Medeiros; ALVAREZ-LAJONCHERE, Luis; CERQUEIRA, Vinicius Ronzani and CANDIDO, Sidinei. Validation of an ovarian biopsy method for monitoring oocyte development in the fat snook, Centropomus parallelus Poey, 1860 in captivity. Braz. arch. biol. technol. [online]. 2004, vol.47, n.4, pp.643-648. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132004000400018.
The validation of an ovarian biopsy method for in vivo assessment of oocyte maturation in Centropomus parallelus was studied. Diameters of intra-ovarian oocytes siphoned with cannula were analyzed fresh and preserved with 1% formalin in 0.7% NaCl solution. Oocytes in different stages were present along the ovaries, up to the tertiary yolk globule stage, which had a unimodal diameter frequency distribution. The oocyte diameter means were not significantly different at four sites along the ovaries (P > 0.05). Samples obtained with cannula were representative of the ovary central portion, in vivo and in vitro samples of the seven females examined were not significantly different (P > 0.05). An estimate of the coefficient of variation corrected for bias (P < 0.05) for 8 repeated in vivo samples was 1.9 ± 0.6. The results demonstrated that for the species, the biopsy method was satisfactory, providing representative samples of the ovaries.
Keywords : Ovarian Biopsy; Fat Snook; Centropomus parallelus.