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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913On-line version ISSN 1678-4324

Abstract

SARUBBO, Leonie Asfora et al. Partition of proteins in aqueous two-phase systems based on Cashew-nut tree gum and poly(ethylene glycol). Braz. arch. biol. technol. [online]. 2004, vol.47, n.5, pp.685-691. ISSN 1516-8913.  http://dx.doi.org/10.1590/S1516-89132004000500003.

The partitioning of two proteins, bovine serum albumin (BSA) and trypsin was studied in an aqueous poly(ethylene glycol)(PEG)- Cashew-nut tree gum system. The phase diagram was provided for Cashew-nut tree gum and PEG molecular weight of 1500 at two different temperatures. The influence of several parameters including concentrations of polymers, pH, salt addition and temperature on the partitioning of these proteins were investigated.. The results of this research demonstrated the importance of the protein characteristics for partitioning in aqueous biphasic system.

Keywords : Aqueous two-phase systems; ine serum albumin; Cashew-nut tree gum; protein partition.

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