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Brazilian Archives of Biology and Technology

versão impressa ISSN 1516-8913

Resumo

RAFFOUL, Tássia et al. Performance evaluation of CHO-K1 cell in culture medium supplemented with hemolymph. Braz. arch. biol. technol. [online]. 2005, vol.48, n.spe, pp. 85-95. ISSN 1516-8913.  http://dx.doi.org/10.1590/S1516-89132005000400011.

The aim of this work was to evaluate the potential of hemolymph utilization as a culture medium supplement to cultivate the animal cell CHO-K1. For this purpose 1% v/v of hemolymph was added to DMEM medium containing 10% v/v of FBS and 1 or 4.5 g/L of glucose. The culture was grown in spinner flasks incubated in a 10% v/v CO2 environment, at 37ºC, with the Cytodex 1 microcarrier. Comparing the results obtained from the culture with hemolymph against those without hemolymph, a positive influence of the hemolymph was observed, as the experiment with hemolymph presented a 52% higher cell concentration and a higher productivity of up to 40%.

Palavras-chave : Animal cell; CHO-K1; microcarrier; growth; attachment; hemolymph.

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