Due to the need of showing the reliability of results from biological essays, such as in health and environmental areas, laboratories have been implementing quality systems, particularly ABNT NBR ISO/IEC 17025 (ABNT, 2001), which include as one of the requirements, the calculation of uncertainty in essays results. It is of common knowledge that estimating uncertainty in essays using organisms, such as animal and vegetal species, is difficult, preventing from meeting that requirement in the standard. The paper shows the calculation of uncertainties in influences quantities in these essays as a tool to allow validating the measuring process, by using the essays performed by Rhodia Environmental Impact Analysis Laboratory and IPEN Ecotoxicology Laboratory as examples.
Uncertainty; influence quantities; biological essays
