SciELO - Scientific Electronic Library Online

vol.49 issue3Partial purification and characterization of xylanase produced by Penicillium expansumGlucose isomerization in simulated moving bed reactor by Glucose isomerase author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913On-line version ISSN 1678-4324


NEVES, Marcos Antonio das; KIMURA, Toshinori; SHIMIZU, Naoto  and  SHIIBA, Kiwamu. Production of alcohol by simultaneous saccharification and fermentation of low-grade wheat flour. Braz. arch. biol. technol. [online]. 2006, vol.49, n.3, pp.481-490. ISSN 1516-8913.

Two samples of low-grade wheat flour, namely low-grade 1 (LG1) and low-grade 2 (LG2), with different carbohydrate and fibrous content, were used as substrates. The samples were liquefied using various concentrations of a- or b-amylase, in order to optimize the production of fermentable sugars; the enzyme a-amylase revealed higher performance. After liquefaction, the simultaneous saccharification and fermentation was conducted in a jar fermentor. Amyloglucosidase was used for saccharification, and dry baker's yeast, S. cerevisiae, for fermentation simultaneously. Glucose was consumed promptly in both cases, LG1 and LG2; ethanol production was considerably higher in LG1 (38.6 g/L), compared to LG2 (24.9 g/L). The maximum ATP production was observed early in the SSF process. LG1 revealed higher potential as substrate for ethanol production.

Keywords : Low grade; wheat flour; yeast; fermentation; ethanol.

        · abstract in Portuguese     · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License