Brazilian Archives of Biology and Technology
versión impresa ISSN 1516-8913
BEZERRA, Raquel Pedrosa et al. Extraction of amylase from fermentation broth in poly (Ethylene Glycol) salt aqueous two-phase system. Braz. arch. biol. technol. [online]. 2006, vol.49, n.4, pp. 547-555. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132006000500003.
Studies were carried out on the partition of amylase from Bacillus subtilis in a minimal medium at 37 ºC and 110 rpm. Enzyme recovery was carried out in aqueous two-phase system PEG-Phosphate salt were carried out. The best purification factor (5.4) was obtained in system PEG 1000 (16.7% w/w) with potassium phosphate (14.8% w/w), at pH 6.0, resulting in a recovery of 45.2% activity enzymatic in the salt-rich phase.
Palabras llave : Amylases; aqueous two-phase systems; Bacillus subtilis; partition; poly (ethylene glycol).