Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913
REBELLO, Bernardo Machado et al. Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells. Braz. arch. biol. technol. [online]. 2007, vol.50, n.spe, pp. 153-159. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132007000600019.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and perimeter/area ratio determination. P. flavicarpa extract altered (p<0.05) the fixation of 99mTc on plasma proteins and the perimeter/area ratio of red blood cells. Substances present in P. flavicarpa extract could affect the labeling of blood constituents with 99mTc acting in specific targets as membrane of red blood cells.
Keywords : Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; technetium-99m.