Brazilian Archives of Biology and Technology
versión impresa ISSN 1516-8913
KLEMZ, Claudio et al. Cytochrome P450 detection in liver of the catfish Ancistrus multispinis (Osteichthyes, Loricariidae). Braz. arch. biol. technol. [online]. 2010, vol.53, n.2, pp. 361-368. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132010000200015.
Sensitive biological responses to environmental contaminants are useful as early warning signals to predict the damages by long-term exposure. Protocols standardization to quantify biochemical parameters in different fish species is required to validate its use as biomarkers. Comparative studies from different fish species and its interpretation are a challenge for the validation of its use as general biomarkers, representative of environmental impact. In this study, the protocol for liver cytochrome P450 (CYP) analysis from the native Brazilian fish Ancistrus multispinis was established. The microsome contamination by hemoglobin during the analysis of CYP in liver was detected, leading to misinterpretation of the results. The spectrophotometric method for CYP analysis was adapted in order to diminish the hemoglobin interference. Additionally, the western blotting method for CYP1A analysis was tested with success for this fish species.
Palabras llave : cytochrome P450; fish; Ancistrus multispinis; biomarker.