Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913
SALMON, Denise Naomi et al. Formulated products containing a new phytase from Schyzophyllum sp. phytase for application in feed and food processing. Braz. arch. biol. technol. [online]. 2011, vol.54, n.6, pp. 1069-1074. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132011000600001.
A new formulated product containing high yield of phytase from Schizophyllum sp., an important mushroom used for medicinal studies, was developed for application in feed industries and for future use in food processing. The enzyme presented a high activity yield 55.5 U/mL and 6240 U/gds in liquid and solid formulated product, respectively. It showed a good shelf-life in concentrated product, retaining 67.8% of its activity after 60 days of storage at room temperature and 90% of the activity was maintained in the liquid formulation after the same period. Powder bioformulated product maintained 77% of its activity after two months of storage, without the addition of chemical additives, which was named as a new bioformulated product containing high quantities of phytase. After separation and concentration steps, enzyme stability was monitored in two forms: liquid and solid. The liquid product was stable with the presence of manitol and polyethylene glycol at 1% (w/v), while solid product was the most stable product without the presence of chemical additives.
Keywords : phytase; Shizophyllum sp; downstream steps; stability; formulation.