Brazilian Archives of Biology and Technology
versão impressa ISSN 1516-8913
LOPES, Toni Jefferson; YAGINUMA, Sandra Regina; QUADRI, Mara Gabriela Novy e QUADRI, Marintho Bastos. Evaluation of red cabbage anthocyanins after partial purification on clay. Braz. arch. biol. technol. [online]. 2011, vol.54, n.6, pp. 1349-1356. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132011000600022.
The aim of this work was to evaluate the red cabbage anthocyanins quality after purification by static adsorption assays on clay (Tonsil Terrana 580FF). Thin-layer chromatography analysis and scan spectrometry on UV-Visible showed a good dye stability of the pigment interacting with the clay. The use of pH 3.0 buffer during the batch assays provided a protective effect on the cabbage anthocyanins, limiting the dye acid hydrolyses. Analyses of the reducing sugars contents of the extract showed that clay retained 20% average of total free sugars under the test conditions.
Palavras-chave : Thin-layer chromatography; spectrophotometry; purification; batch conditions.