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Brazilian Archives of Biology and Technology
Print version ISSN 1516-8913
RAMOS, Márcio Antônio Gomes and YOSHIOKA, Sergio Akinobu. Bioremediation of herbicide velpar K® in vitro in aqueous solution with application of EM-4 (effective microorganisms). Braz. arch. biol. technol. [online]. 2012, vol.55, n.1, pp. 145-149. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132012000100018.
This work assessed the bioremediation of herbicide Velpar K®, in vitro in aqueous solution, used against weeds in sugar cane in São Paulo state. The herbicide contained Hexazinone and Diuron. It was used the microbial inoculant denominated Effective Microorganisms (EM-4), pool of microorganisms from soil that contained lactic and photosynthetic bacteria, fungi, yeasts and actinomycetes for bioremediation. Results for the depth of cultivation on agar-agar inoculated with EM-4 showed the microorganisms growth in the concentrations between 0.2% and 1.0% of the Velpar K®in the gel. The analysis of high performance liquid chromatography (HPLC) showed that the EM-4 was effective for the bioremediation of the herbicide, which reached the values of 80% for diuron and 70% for hexazinone after 21 days in solution of 2:1 of Velpar K®/EM-4 ratio. These results could be useful for planning the bioremediation of contaminated areas with Velpar K®.
Keywords : Velpar K®; herbicide; bioremediation; effective microorganisms.