Brazilian Archives of Biology and Technology
versión impresa ISSN 1516-8913
SILVA, Gildo Almeida da et al. Rapid yeast DNA extraction by boiling and freeze-thawing without using chemical reagents and DNA purification. Braz. arch. biol. technol. [online]. 2012, vol.55, n.2, pp. 319-327. ISSN 1516-8913. http://dx.doi.org/10.1590/S1516-89132012000200020.
The purpose of this work was to study a rapid yeast DNA extraction by boiling and freeze-thawing processes without using chemical reagents or any purification procedures, to obtain a high grade PCR-product. A specific DNA fragment of the 18S region of Dekkera bruxellensis and Saccharomyces cerevisiae was chosen. The described boiling and freeze-thawing protocols generated the PCR-grade product preparations and could be used to process many samples. The amplification of the fragments could be observed after 30 and 35 cycles. These processes of extraction without using any kind of chemical reagents, especial water, and purification procedures proved to be efficient, reproducible, simple, fast, and inexpensive.
Palabras llave : Boiling; freeze-thawing; DNA extraction; Dekkera bruxellensis; Saccharomyces cerevisiae.