SciELO - Scientific Electronic Library Online

 
vol.55 issue3Comparison between deep intracornual artificial insemination (dIAI) and conventional artificial insemination (AI) using low concentration of spermatozoa in beef cattleDetermination of eye irritation potential of low-irritant products: comparison of in vitro results with the in vivo draize rabbit test author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913

Abstract

CUSTODIO, Luiz Antonio et al. Detection of Lsr2 gene of Mycobacterium leprae in nasal mucus. Braz. arch. biol. technol. [online]. 2012, vol.55, n.3, pp. 375-380. ISSN 1516-8913.  http://dx.doi.org/10.1590/S1516-89132012000300007.

In the present study, nasal mucus from patients with leprosy were analyzed by PCR using specific primers for Lsr2 gene of Mycobacterium leprae. The presence of Lsr2 gene in the nasal mucus was detected in 25.80% of patients with paucibacillari leprosy, and 23.07% of contacts. Despite the absence of clinical features in the contact individuals, it was possible to detect the presence of Lsr2 gene in the nasal mucus of these individuals. Therefore, PCR detection of M. leprae targeting Lsr2 gene using nasal mucus samples could contribute to early diagnosis of leprosy.

Keywords : Mycobacterium leprae; leprosy; hanseniase; nasal mucus; PCR.

        · text in English     · pdf in English