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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913


NOBREGA, Andréa Martins da et al. Determination of eye irritation potential of low-irritant products: comparison of in vitro results with the in vivo draize rabbit test. Braz. arch. biol. technol. [online]. 2012, vol.55, n.3, pp.381-388. ISSN 1516-8913.

In an attempt to build the evaluation strategies to assess the human eye irritation, a reassessment of some in vitro tests is necessary, particularly concerning the non-irritants, mild and moderate irritants. Thus, the correlations between results obtained from the Draize test with the in vitro methods HET-CAM (Hens Egg Test-Chorion Allantonic Membrane) and RBC haemolysis assay to assess the ocular irritancy potential of 20 eye drops were examined. Parameters such as accuracy (%) and specificity (%) were determined. All results were correlated with RBC correctly with the results obtained in the Draize test. The HET-CAM presented four false-positive results, showing a tendency of data overestimation. Despite the high specificity provided mainly by the RBC, it would be necessary to test a wider range of products representing all the scales of irritation to confirm its ability to be used as a first alternative to test products that could be presumptive non-irritants.

Keywords : Alternative methods; eye irritation; Draize Test; low-irritant; HET-CAM; RBC.

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