SciELO - Scientific Electronic Library Online

vol.55 issue3Interannual and seasonal variability of macroinvertebrates in monsoonal climate streamsTolerance of Anadenanthera peregrina to Eucalyptus camaldulensis and Eucalyptus grandis essential oil as condition for mixed plantation author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Archives of Biology and Technology

On-line version ISSN 1678-4324


HASBULLAH, Nor Azlina; TAHA, Rosna Mat; SALEH, Azani  and  MOHAMED, Normadiha. Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation. Braz. arch. biol. technol. [online]. 2012, vol.55, n.3, pp.411-416. ISSN 1678-4324.

In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f. Biochemical changes in chlorophyll and soluble protein content of pre- and post- irradiated Gerbera callus were studied. Non-irradiated callus demonstrated the highest amount of chlorophyll content as compared to callus irradiated at 10, 20, 30, 40, 50 and 60 Gy. In addition, the amount of chlorophyll b was relatively higher than chlorophyll a in both the irradiated and non-irradiated callus, except for callus irradiated at 10 Gy. Biochemical differentiation based on total soluble protein content revealed gradual reduction after day 9 of exposure to gamma irradiation. Reduction of soluble protein content was observed in all the treatments as the increase of incubation period.

Keywords : Gerbera jamesonii; gamma irradiation; callus; in vitro; chlorophyll; soluble protein.

        · text in English     · English ( pdf epdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License