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Brazilian Archives of Biology and Technology
On-line version ISSN 1678-4324
TSAI, S. and LIN, C.. Advantages and Applications of Cryopreservation in Fisheries Science. Braz. arch. biol. technol. [online]. 2012, vol.55, n.3, pp.425-434. ISSN 1678-4324. http://dx.doi.org/10.1590/S1516-89132012000300014.
Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic cells is another option for preserving both maternal and paternal genome. In this paper, an overview of the current state of aquatic species is followed by a discussion on the sperm, embryos, oocytes and embryonic cells - blastomeres.
Keywords : cryopreservation; sperm; embryo; oocyte; blastomere.