SciELO - Scientific Electronic Library Online

 
vol.57 issue2Extracellular methionine amino peptidase (MAP) production by Streptomyces gedanensis in solid-state fermentationEffects of GDF-9 and FSH on mRNA expression for FSH-R, GDF-9 and BMPs in in vitro cultured goat preantral follicles author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

Share


Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913

Abstract

ISMAIL, Mohd Iswadi et al. Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability. Braz. arch. biol. technol. [online]. 2014, vol.57, n.2, pp.194-199. ISSN 1516-8913.  http://dx.doi.org/10.1590/S1516-89132014000200006.

The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25π x 278 mm2) and single tubing-type canister (4π x 90 mm2). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant changes were found in sperm kinetics (VCL, VAP, VSL) of tubing-type canister compared to commercial canister. No significant changes in the motility and viability of the bull sperm at the top of tubing-type canister and commercial canister. There were no significant changes in sperm progression (LIN, WOB, PROG) in both the canisters. Developed tubing-type canister in this study showed potential as an alternative to be used in bull sperm cryo-storage.

Keywords : Cryopreservation; sperm; canister; motility; viability.

        · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License