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Brazilian Archives of Biology and Technology

Print version ISSN 1516-8913


SOUSA, Marylane de et al. Crude glycerol from biodiesel industry as substrate for biosurfactant production by Bacillus subtilis ATCC 6633. Braz. arch. biol. technol. [online]. 2014, vol.57, n.2, pp.295-301. ISSN 1516-8913.

Glycerol, a co-product of the biodiesel industry, may be a suitable raw material for the production of high added-value compounds by the microorganisms. This study aimed to use the glycerol obtained from the biodiesel production process as the main carbon source for biosurfactant production by Bacillus subtilis ATCC 6633. Results indicated that the strain lowered the surface tension of the cell-free fermented broth to 31.5 ± 1.6 mN/m, indicating the production of biosurfactant. The critical micelle concentration (CMC = 33.6 mN/m) obtained was similar to the previously reported for biossurfactants isolated from other Bacillus. The produced biosurfactant was able to emulsify n-hexadecane and soybean oil.

Keywords : Biosurfactant; Biodiesel; Glycerol and Surfactin.

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