The Tc1/Mariner sequence was isolated and mapped on chromosomes aiming to verify the association of this transposable element (TE) and chromosomal rearrangements in Rineloricaria. Cytogenetic analysis showed that Tc1/Mariner does not co-localize with chromosomal fusion points, in addition the analysis revealed intense molecular degeneration in its DNA sequence.
Key words:
chromosome rearrangement; double-FISH; transposon
| Rineloricaria individuals | TE description | TE nucleotide sequence position | Nucleotide position of the reference TE (CENSOR) | Categories | Degree of DNA similarity |
|---|---|---|---|---|---|
| R. latirostris | TC1DR3 | 1-555 | 1-601 | DNA | 0.8217 |
| TC1DR3 | 573-1081 | 668-1225 | DNA | 0.8031 | |
| R. pentamaculata | TC1DR3 | 13-310 | 89-418 | DNA | 0.7855 |
| TC1 FR1 | 331-619 | 652-951 | DNA/Mariner | 0.7840 | |
| Penelope-5 XT | 641-680 | 2807-2846 | NonLTR/Penelope | 0.8780 | |
| Penelope-6 XT | 718-781 | 2491-2553 | NonLTR/Penelope | 0.8000 | |
| Ruka | 844-905 | 6-67 | NonLTR/SINE/SINE2 | 0.7258 | |
| L2-2 SSa | 969-1017 | 3984-4032 | NonLTR/L2 | 0.8776 | |
| TC1DR3 | 1018-1183 | 1019-1214 | DNA | 0.8070 | |
| R. stellata | TC1DR3 | 1-133 | 1084-1225 | DNA | 0.8074 |
| TC1DR3 | 157-348 | 52-308 | DNA | 0.8477 | |
| Helitron-2 DR | 361-428 | 2890-2960 | DNA/Helitron | 0.7183 | |
| DNA-4-2 NV | 678-752 | 323-386 | DNA | 0.7794 | |
| hat-N2 AP | 760-870 | 582-688 | DNA/hat | 0.7570 | |
| DNA8-74 AP | 910-1011 | 119-212 | DNA | 0.7753 | |
| Helitron-N2 XT | 1242-1285 | 1257-1299 | DNA/Helitron | 0.8409 | |
| RC1 FR1 | 1290-1326 | 1184-1220 | DNA/Mariner | 0.8378 | |
| R. capitonia | TC1DR3B | 2-54 | 837-889 | DNA/Mariner | 0.8679 |
| TC1DR3 | 113-670 | 1-752 | DNA | 0.8462 |
