Revista Brasileira de Ciências Farmacêuticas
Print version ISSN 1516-9332
SQUINA, Fabio M. and MERCADANTE, Adriana Z.. HPLC analysis of carotenoids from five Rhodotorula strains. Rev. Bras. Cienc. Farm. [online]. 2003, vol.39, n.3, pp.309-318. ISSN 1516-9332. http://dx.doi.org/10.1590/S1516-93322003000300010.
A method for extraction and HPLC separation of carotenoids from fiveRhodotorula strains was optimized. The extraction by mechanical disruption of the yeast cell wall with fine treated sand was shown to be more efficient than chemical disruption with dimethylsulfoxide. The carotenoids were separated and quantified by HPLC on a C18 column using as mobile phase acetonitrile/methanol (0.1% triethylamina)/ethyl acetate (75:15:10) with 100% methanol (0.1% triethylamine) between the injections, at a flow rate of 1.0 mL/min. In all strains, the major carotenoids found were torularhodin, torulene, ³-carotene and ²-carotene. The total carotenoid contents, in µg/g, obtained were 251.7 for R. glutinis,123.5 for R. rubra,113.2 for R. araucariae,105.8 for R. lactosaand 103.7 for R. minuta.
Keywords : Carotenoids; Rhodotorula; HPLC.