Revista Brasileira de Ciências Farmacêuticas
versão impressa ISSN 1516-9332
LIMA, Dione Marçal; NUNES JUNIOR, Guilhermino Pereira; FERRI, Pedro Henrique e SANTOS, Suzana Costa. Determination of fluconazole in serum and amniotic fluid of rats by gas-chromatography/mass spectometry (GC/MS). Rev. Bras. Cienc. Farm. [online]. 2005, vol.41, n.2, pp. 223-227. ISSN 1516-9332. http://dx.doi.org/10.1590/S1516-93322005000200010.
Rats treated with oral dose of 100 mg/kg of fluconazole during pregnancy had their serum and amniotic fluid quantified for this drug using a GC/MS method. Fluconazole was extracted with ethyl acetate from samples and analysed by a GC-MS Shimadzu QP5050A system using a CBP-5 fused silica capillary column. Tioconazole was used as internal standard. Calibration curve was linear within the range 10.0 - 300.0 µg/mL. The limit of quantification was 0.1 µg/mL and no interference was observed in the blank serum and amniotic liquid. The mean concentrations of the drug in the serum and amniotic fluid were 206.01 ± 105.25 µg/mL and 125.34 ± 65.24 mg/mL, respectively. This procedure showed to be sensitive and efficient enough for the use in teratogenic studies of fluconazole and other azole drugs.
Palavras-chave : Fluconazole; GC/MS; Serum; Amniotic fluid.