SciELO - Scientific Electronic Library Online

vol.41 número4In vitro biopharmaceutical evaluation of solid dosage forms containing doxycyclineDetection of androgenic anabolic steroids (AAS) in urine samples by gas chromatography-mass spectrometry índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados




Links relacionados


Revista Brasileira de Ciências Farmacêuticas

versión impresa ISSN 1516-9332


LARA, Marilisa Guimarães et al. Preparation and scaling up of a low phenylalanine enzymatic hydrolysate of bovine whey proteins. Rev. Bras. Cienc. Farm. [online]. 2005, vol.41, n.4, pp.459-466. ISSN 1516-9332.

We describe the preparation of pancreatic enzymes hydrolysate of milk whey proteins containing low levels of aromatic amino acids. Pancreatin and trypsin/chymotrypsin (6.3% w/w protein) when used to hydrolyze whey proteins for 27 h at 37±2 ºC, released 74% of the Phe, 100% of the Tyr and 100% of the Trp as free amino acids. Most of the free aromatic amino acids present in 2 kg hydrolysate were separated from the remaining peptides and other amino acids by gel filtration on a 15 liter Sephadex G-25 column eluted with 5% acetic acid at 60 liters h-1 at 25ºC. The product, recovered in 37% yield, contained 0.70 mmol Phe, 0.41 mmol Tyr, and <0.01 mmol Trp/100 mmol recovered amino acids. The hydrolysate had a general amino acid composition similar to the whey proteins from which it was prepared and could be used as a nitrogen source for patients with phenylketonuria or tyrosinemia after the addition of appropriate aromatic amino acids.

Palabras clave : Low phenylalanine diet; Phenylketonuria; Tyrosinemia; Whey protein hydrolysates; Pancreatin hydrolysate; Gel filtration.

        · resumen en Portugués     · texto en Inglés     · Inglés ( pdf epdf )


Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons