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Revista Brasileira de Ciências Farmacêuticas

Print version ISSN 1516-9332


PAOLINELLI, Sonia Torquato; REEN, Rashmeet  and  MORAES-SANTOS, Tasso. Curcuma longa ingestion protects in vitro hepatocyte membrane peroxidation. Rev. Bras. Cienc. Farm. [online]. 2006, vol.42, n.3, pp.429-435. ISSN 1516-9332.

The goal of the present study was to evaluate the effect of turmeric ingestion on lipid peroxidation and GSH content, promoted by in vitro acetaminophen, on hepatocytes primary culture from well-nourished and malnourished rats. Four groups of Holtzman male rats were used: 1) WNG, well-nourished, fed lab chow diet ad libitum; 2) MNG, malnourished, fed 60% of the diet consumed by WNG; 3) WNG+T fed the same diet of WNG, but containing 1% of turmeric; 4) MNG+T fed 60% of the diet consumed by WNG+T. The animals were sacrificed at 90 days of age, the livers excised and hepatocytes primary cultures were prepared. Half of the plates of hepatocytes culture were treated with acetaminophen. Dose-response curve showed that 6 mM acetaminophen increased peroxidation around 54% and decreased GSH content around 63%. The model of malnutrition used, by restricting food ingestion (40%), decreased body weight in 33% and peroxidation index around 42% and increased GSH content around 43%. Turmeric ingestion decreased hepatocyte peroxidation in both well-nourished (42%) and malnourished rats (33%) and was able to avoid the acetaminophen pro-oxidant effect in both well-nourished and malnourished animals. Turmeric ingestion played a beneficial role to the organism and, therefore, can be considered a functional food.

Keywords : Turmeric; Acetaminophen; Food restriction; Lipid peroxidation; Hepatocytes primary culture.

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