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Revista Brasileira de Ciências Farmacêuticas

Print version ISSN 1516-9332

Abstract

PARISOTTO, Graciele; FERRAO, Marco Flôres; FURTADO, João Carlos  and  MOLZ, Rolf Fredi. Determination of amoxicillin content in powdered pharmaceutical formulations using DRIFTS and PLS. Rev. Bras. Cienc. Farm. [online]. 2007, vol.43, n.1, pp. 89-96. ISSN 1516-9332.  http://dx.doi.org/10.1590/S1516-93322007000100011.

The amount of amoxicillin in pharmaceutical formulations was determined using spectra of diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), in association with partial least squares (PLS) regression. The spectra of 24 different samples (17 for the calibration set and 7 for the validation set), which had been registered using a Nicolet Magna 550 spectrophotometer, contained 76.7-94.3% of amoxicillin in starch. The PLS models were constructed with auto-scaled or variance-scaled data, and multiplicative scatter correction (MSC). The best model presented R2=0.9936, RMSEC=0.441 and RMSEV=0.790. The analytical method proposed is non-destructive and the cost and time of analysis are very much reduced, allowing for the fast and direct determination of amoxicillin content.

Keywords : Multivariate regression; Partial least squares regression; Diffuse reflectance infrared Fourier transform spectroscopy; Chemometrics.

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