Revista Brasileira de Ciências Farmacêuticas
versão impressa ISSN 1516-9332
LOPES, Patrícia Santos et al. In vitro safety assessment of papain on human skin: A qualitative Light and Transmission Electron Microscopy (TEM) study. Rev. Bras. Cienc. Farm. [online]. 2008, vol.44, n.1, pp.151-156. ISSN 1516-9332. http://dx.doi.org/10.1590/S1516-93322008000100017.
Papain is a thiol proteolytic enzyme widely used in dermatology that found applications in wound treatment. Recently, papain was also used as absorption enhancer which can modify the peptide/protein material in the bilayer domain. We investigated papain safety using human skin that was exposed to papain in vitro at different times: 4, 24 and 48 hours. The samples were examined using Light and Transmission Electron Microscopy (TEM) to study of the mechanisms involved in enhancer-skin interaction. After 24 hours, changes occurred in corneosomes. However, samples of 48 hours did not show major changes in agreement with the control. These findings indicated that papain could be used safely onto the skin.
Palavras-chave : Transmission electron microscopy [qualitative analysis]; Light microscopy [qualitative analysis]; Stratum corneum; Papain [cosmetic use]; Human skin [cosmetology].