Determination of Di(2-ethylhexyl phthalate) released in the blood from PVC line during the hemodialysis procedure

Fernandes, Beatriz Luci; Maria, Fernanda; Nakao, Lia Sumie; Ingberman, Max; Cubas, Marcia Regina

doi:10.1590/S1517-707620170001.0282

Acessibilidade / Reportar erro

Brasil

Español English

sumário « anterior atual seguinte »

Sumário

Artigo • Matéria (Rio J.) 23 (1) • 2018 • https://doi.org/10.1590/S1517-707620170001.0282 copy

Determination of Di(2-ethylhexyl phthalate) released in the blood from PVC line during the hemodialysis procedure

Determinação de Di(2-etilhexil ftalato) liberado no sangue por linhas de PVC durante o procedimento de hemodiálise

Authorship SCIMAGO INSTITUTIONS RANKINGS

ABSTRACT

The objective of the work is to demonstrate the applicability of the High-Performance Liquid Chromatography (HPLC) on the quantification of the Di(2-ethylhexyl phthalate) (DEHP) released from the Polyvinyl Chloride (PVC) line into the circulating blood during the hemodialysis procedure. As the proposal is not to validate the method, blood samples from five patients and three healthy volunteers were collected, and the plasmas were isolated, prepared and analyzed. The average concentration of DEHP in the patient's plasma was 0.19±0.12 mg/kg of body mass, while in the plasma of the healthy volunteers was 0.003±0.002 µg/kg of body mass. The results showed that the method presented is an alternative to quantify the DEHP in the blood and could assist in the search for alternative materials to apply to extracorporeal circulation equipment. The study was performed according to the national and international standards of ethics in research involving human beings (CEP PUCPR Prot. n.5802).

Keywords:
Renal dialysis; Chronic toxicity; DEHP

Patient/Session	Reuse number	Heparin dose in the circuit (UI)	Blood flow (ml/min)	Period of hemodialysis therapy
P1/1 P1/2 P1/3	0 2 4	5,000	300	3 years and 3 months
P2/1 P2/2 P2/3	5 7 9	without heparin^* * Patient candidate for renal transplant. Reuse number 0 means first use.	300	5 years
P3/1 P3/2 P3/3	9 11 13	6,000	300	3 year and 6 months
P4/1 P4/2 P4/3	0 2 4	7,000	350	4 years and 4 months
P5/1 P5/2 P5/3	11 13 15	8,000	350	4 years and 9 months

Day of analysis	Linear regression	Determination coefficient (r²)	Volunteer / session	Area under the peak (x)
1	y=3.10⁻⁸x – 4.10⁻⁵	0.9974	P4 / 1 initial P4 /1 final P4 / 2 initial P4 / 2 final P4 / 3 initial P4 / 3 final P5 / 1 initial P5 / 1 final P5 / 2 initial P5 / 2 final	3,576.0 20,223.8 2,332.8 2,480.0 4,931.2 6,680.0 34,129.6 43,718.4 7,603.2 7,740.8
2	y=2.10⁻⁸x + 2.10⁻⁵	0.9990	P5 / 3 initial P5 / 3 final	3,803.2 23,950.4
3	y=2.10⁻⁸x + 6.10⁻⁶	0.9913	P1 / 1 initial P1 / 1 final P1 / 2 initial P1 / 2 final P1 / 3 initial P1 / 3 final	5,817.6 6,905.6 6,240.0 6,649.6 1,292.8 4,763.2
4	y= 3.10⁻⁸x+ 8.10⁻⁵	0.9954	P2 / 1 initial P2 / 1 final P2 / 2 initial P2 / 2 final P2 / 3 initial P2 / 3 final	6,428.8 14,163.2 5,017.6 12,294.4 7,878.4 8,601.6
5	y= 3.10⁻⁸ x + 2.10⁻⁵	0.9989	P3 / 1 initial P3 / 1 final P3 / 2 initial P3 / 2 final P3 / 3 initial P3 / 3 final	8,726.4 9,568.1 6,364.8 10,486.4 4,528.8 9,740.8
6	y= 2.10⁻⁸ x – 2.10⁻⁵	0.9994	V1 V2 V3	2,750.4 1,081.6 1,604.8

Patient/session	Concentration of DEHP (μg/ml) 15 min after the session has started	Concentration of DEHP (μg/ml) 15 min before the session has finished	DEHP accumulated per patient, ΔDEHP_m (μg/ml) ± SD	VC (%) for ΔDEHP_m
P1 / 1 P1 / 2 P1 / 3	0.122^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.131^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.032^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.144^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.139^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.101^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.033±0.030	96,83
P2 / 1 P2 / 2 P2 / 3	0.273 0.231 0.316	0.505 0.449 0.338	0.157±0.120	74.63
P3 / 1 P3 / 2 P3 / 3	0.242 0.171^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.101^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.267 0.296 0.202	0.083±0.252	62.40
P4 / 1 P4 / 2 P4 / 3	0.103^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.029^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.108^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.567 0.034^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.161^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	0.174±0.231	144,99
P5 / 1 P5 / 2 P5 / 3	0.984 0.188^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.096^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient.	1.272 0.192^* Samples with * indicate concentration below the limit of quantification (< 0.197 µg/ml); SD = Standard Desviation; VC = Variation Coeficient. 0.499	0.232±0.205	88.64

Patient/Session	ΔDEHP (μg/ml)	Blood flow (ml/min)	ΔDEHP x flow (µg/min)	MDEHP per session (mg)	MDEHPm (mg) ± SD
P1 / 1 P1 / 2 P1 / 3	0.022 0.008 0.069	300	6.60 2.40 20.70	– – –	–
P2 / 1 P2 / 2 P2 / 3	0.232 0.218 0.022	300	69.60 65.40 6.60	16.70 15.69 1.58	11.32±8.45
P3 / 1 P3 / 2 P3 / 3	0.025 0.125 0.053	300	7.50 37.50 15.90	1.80 - -	–
P4 / 1 P4 / 2 P4 / 3	0.464 0.005 0.523	350	162.40 1.75 183.05	– – –	–
P5 / 1 P5 / 2 P5 / 3	0.287 0.004 0.403	350	100.45 1.40 141.05	24.11 - -	–

Patient	MDEHP_m (mg)	Body weight (kg)	MDEHP m/K (mg/kg)
P2	11.32±8.45	60.0±0.05	0.19±0.14
P3	1,80±0,00	71.5±0.05	0.07±0.05
P4	27.78±23.81	70.0±0.05	0.39±0.34
P5	19.43±17.24	75.5±0.05	0.26±0.23

Volunteer	DEHP (μg/ml)	MDEHPm (μg)	Body weight	DEHPm (μg/kg)
V1	0.035	0.35	60.20±0.05	0.006±0,001
V2	0.002	0.02	54.50±0.05	0.000±0.001
V3	0.012	0.12	56.40±0.05	0.002±0.001

Laboratório de Hidrogênio, Coppe - Universidade Federal do Rio de Janeiro, em cooperação com a Associação Brasileira do Hidrogênio, ABH2 Av. Moniz Aragão, 207, 21941-594, Rio de Janeiro, RJ, Brasil, Tel: +55 (21) 3938-8791 - Rio de Janeiro - RJ - Brazil
E-mail: revmateria@gmail.com

Acompanhe os números deste periódico no seu leitor de RSS

[1] Autor Responsável: Beatriz Luci Fernandes