SciELO - Scientific Electronic Library Online

vol.31 issue4Isolation, identification and physiological study of Lactobacillus fermentum LPB for use as probiotic in chickensGenetic diversity of the VP1/VP2 gene of canine parvovirus type 2b amplified from clinical specimens in Brazil author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Brazilian Journal of Microbiology

Print version ISSN 1517-8382
On-line version ISSN 1678-4405


FLATSCHART, Roberto B.  and  RESENDE, Maurício. Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus. Braz. J. Microbiol. [online]. 2000, vol.31, n.4, pp.308-311. ISSN 1517-8382.

Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.

Keywords : Aujeszky’s disease virus; polymerase chain reaction; dot-blot hybridization.

        · abstract in Portuguese     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License