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Brazilian Journal of Microbiology
On-line version ISSN 1678-4405
FLATSCHART, Roberto B. and RESENDE, Maurício. Acute and latent infection in mice with a virulent strain of Aujeszkys disease virus. Braz. J. Microbiol. [online]. 2000, vol.31, n.4, pp. 308-311. ISSN 1678-4405. http://dx.doi.org/10.1590/S1517-83822000000400013.
Acute and latent infections with the Brazilian LA031 strain of Aujeszkys disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
Keywords : Aujeszkys disease virus; polymerase chain reaction; dot-blot hybridization.