SciELO - Scientific Electronic Library Online

vol.32 issue4Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substratesSalmonella in Brazilian and imported pet reptiles author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




Related links


Brazilian Journal of Microbiology

Print version ISSN 1517-8382On-line version ISSN 1678-4405


SCARCELLI, Eliana et al. Comparison of electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni isolated from different animal species. Braz. J. Microbiol. [online]. 2001, vol.32, n.4, pp.286-292. ISSN 1517-8382.

Electrophoretic protein profiles of Campylobacter jejuni subsp. jejuni strains isolated from feces of seven animal species, including man, were compared. Fourteen strains (two from each species) plus two human strains and the reference one, were ruptured by ultrasound and their total soluble proteins were analyzed by SDS-PAGE technique in a 12% polyacrylamide gel with computerized densitometric reading by the molecular analyst software. All the strains had bands in common that correspond to 45 and 66 Kda molecular weight. The disagreement corresponded to a 97 to 200 Kda molecular weight region. From the 17 strains, 13 (76.5%), were classified as biotype I, three (17.6%) as biotype II and one (5.8%) as biotype III. Since protein extracts were obtained from cells grown under identical conditions, and thus, able to express the same phenotype, this disagreement region could be related to different genotypes or serotypes.

Keywords : Campylobacter jejuni; SDS-PAGE; biotyping.

        · abstract in Portuguese     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License