Brazilian Journal of Microbiology
versión impresa ISSN 1517-8382
versión On-line ISSN 1678-4405
BARENSE, Renata I.; CHELLEGATTI, Maria Angélica dos S.C.; FONSECA, Maria José V. y SAID, Suraia. Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans. Braz. J. Microbiol. [online]. 2001, vol.32, n.4, pp.327-330. ISSN 1517-8382. http://dx.doi.org/10.1590/S1517-83822001000400014.
Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
Palabras clave : Exopolygalacturonase; pectinase; polygalacturonase; Penicillium frequentans.