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Brazilian Journal of Microbiology

versão impressa ISSN 1517-8382versão On-line ISSN 1678-4405

Resumo

ANDRADE, Vânia Sousa et al. Production of extracellular proteases by Mucor circinelloides using D-glucose as carbon source / substrate. Braz. J. Microbiol. [online]. 2002, vol.33, n.2, pp.106-110. ISSN 1517-8382.  http://dx.doi.org/10.1590/S1517-83822002000200002.

Recently, some Mucorales species have been reported as protease producers. The production of extracellular proteases by Mucor circinelloides using glucose as substrate was studied. Experiments were carried out with different D-glucose concentrations (40, 60 and 80 g/L). Biomass, pH and protease activity were determined. Although biomass production had reached best yields for the medium containing D-glucose in a concentration of 80 g/L, the enzymatic production was higher when the substrate concentration was reduced to 40 g/L. The yield factor for product on cell growth and the yield factor for product on carbon substrate were higher when the microorganism grew in medium containing 40 g/L glucose. The kinetics parameters suggest that this strain seems to be promising as an alternative microorganism for protease production.

Palavras-chave : extracellular proteases; D-glucose; substrate; Mucor circinelloides.

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